Calcium Activation of the Androgen Receptor in Prostate Cells

Figure 2

Effects of calcium in androgen receptor transfected HEK293T cells. HEK293T cells were plated in lipoic acid free and phenol red free IMEM containing 5% CCS for 24 hours and transiently transfected with the androgen receptor plasmid (2.5 μg/μl). After 24 hours, the medium was diluted to 1% CCS and the cells were treated with dihydroxytestosterone (DHT, 5 nM), R1881 (5 nM), or calcium (Ca₊₂, 1 mM and 3 mM) in the absence and the presence of the second-generation antiandrogen enzalutamide (ET, 10 μM). RNA was isolated, and the amount of WNT7B (a) and NKX3.1 (b) mRNA was measured using a qRT-PCR assay and normalized to the amount of 18S rRNA. Data are presented as fold change (mean ± SEM; biological replicates = 3; technical replicates = 3; and , , , , ).

(a)

(b)