Single-Cell RNA Sequencing Analysis of Steroidogenesis and Spermatogenesis Impairment in the Testis of db/db Mice

Figure 1

The histopathological characteristics of testis in db/db mice. (a) Testis tissues from four C57BL/6J mice (CON) and four db/db mice (db/db) were stained with hematoxylin and eosin. The red arrow indicates a Leydig cell, and the yellow arrow indicates a Sertoli cell. The green arrow shows a spermatid, which together with spermatogonia and spermatocyte are used to count the total number of cells in the tubule. The wall thickness of the tubule was measured at 3 locations in each picture (blue lines). (b) Cross-sectional area (CSA) of seminiferous tubules, the thickness of the cell layer of seminiferous tubules, and total cell number, Sertoli cells, and Leydig cells number per square millimeter in CON and db/db groups.

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