Immobilization of HRP Enzyme on Layered Double Hydroxides for Biosensor Application

Figure 3

(a) Cyclic voltammogram of gold electrode functionalized with HRP before and after the injection of different H₂O₂ concentration. (b) The chronoamperometry curve of functionalized gold electrode with HRP at a fixed potential of 350 mV after H₂O₂ injections. (c) Current variation (current after H₂O₂ injection) of the developed biosensor after different injection of H₂O₂ concentration at 350 mV obtained from Figure 3(a) in PBS buffer and raw milk. Linear regression was observed between 0 and 200 μM.

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