Research Article
Electrochemical Analysis of Ultrathin Polythiolsiloxane Films for Surface Biomodification
Figure 3
PMPMS thiols remaining after crosslinking support immobilization of disulfide-terminated probes through thiol/disulfide exchange reactions, leading to hybridization signal (peak at around 0.5 V) for TMEA and BM(PEG)3 crosslinked films. Lack of signal in BMH and BMDPM treated films indicates near complete conversion of PMPMS thiols. Conditions of PMPMS deposition: 10 mM PMPMS in DMF, 2 hrs. PMPMS crosslinking: 1 mM crosslinker in DMF, 17 hrs.