Optimization of Lactic Acid Production from Pineapple By-Products and an Inexpensive Nitrogen Source Using Lactiplantibacillus plantarum strain 4O8

Table 3

Experimental design for the optimization process of lactic acid production.

Run	Coded levels			Real levels			Lactic acid (g/l)
				Nitrogen source (ml/l) ()	Temperature (°C) ()	Carbone source (g/l) ()	Experimental values	Predicted values
1	1	-1	-1	90	25	300	17.21 ± 0.00	17.11
2	-1	-1	-1	30	25	300	16.75 ± 0.01	17.77
3	0	0	0	60	32.5	550	17.60 ± 0.01	17.64
4	1	-1	1	90	25	800	15.52 ± 0.01	16.32
5	0	0	0	60	32.5	550	17.59 ± 0.01	17.64
6	-1	1	-1	30	40	300	14.65 ± 0.02	13.52
7	-1	-1	1	30	25	800	17.22 ± 0.02	17.53
8	1	1	1	90	40	800	17.27 ± 0.01	15.93
9	0	0	0	60	32.5	550	16.95 ± 0.02	17.64
10	0	0	0	60	32.5	550	17.10 ± 0.00	17.64
11	-1	1	1	30	40	800	15.55 ± 0.02	15.33
12	1	1	-1	90	40	300	15.30 ± 0.03	14.66
13	0	0	-1.633	60	32.5	141.75	17.55 ± 0.00	17.92
14	0	0	0	60	32.5	550	17.00 ± 0.00	16.22
15	0	0	0	60	32.5	550	16.90 ± 0.00	16.22
16	0	-1.633	0	60	20.25	550	11.43 ± 0.03	10.03
17	0	1.633	0	60	44.75	550	4.36 ± 0.02	6.24
18	1.633	0	0	109	32.5	550	17.22 ± 0.02	17.85
19	0	0	1.633	60	32.5	958.25	18.64 ± 0.01	18.76
20	-1.633	0	0	11.01	32.5	550	18.04 ± 0.01	17.89

: nitrogen source; : temperature; : carbon source.