International Journal of Food Science

Research Article

Purification, HR-LC-ESI-MS-MS Identification, and Peptide Prediction of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from Chanos chanos

Table 1

BLIS activity, BLIS specific activity and purification level of selected BLIS-gel filtration fraction.


BLIS-GF fraction	Protein (μg mL^-1)	BLIS activity (AU mL^-1)				BLIS-specific activity (AU μg^-1)				Purification fold
BLIS-GF fraction	Protein (μg mL^-1)	EC	SA	LM	ST	EC	SA	LM	ST	EC	SA	LM	ST

A-S5-3	15.7	9098.7	10029.8	13786	10283.5	580.6^d,A	640.0^f,AB	879.7^g,C	656.2^i,B	4.0	5.8	3.3	3.9
A-S8-2	11.9	8411.1	5536.5	7354	6312.4	709.1^e,D	466.7^d,A	619.9^f,C	532.1^gh,B	1.9	3.3	2.3	1.4
A-S8-5	14.7	7809.4	6067.7	5769.6	3541.6	530.5^d,B	412.2^d,C	392.0^e,B	240.6^e,A	1.4	2.9	1.5	0.6
A-S11-2	14.3	7178.7	8090.1	5219.7	4589.1	500.3^cd,B	563.8^e,B	363.8^e,A	319.8^f,A	1.6	5.2	1.4	1.1
A-S11-3	15.9	6280.2	5536.5	1759.7	1284.1	394.1^b,B	347.4^c,B	110.4^a,A	80.6^b,A	1.3	3.2	0.4	0.3
A-S11-7	17.9	7325.3	6067.7	5255.9	3226.2	409.4^b,C	339.1^c,BC	293.7^d,B	180.3^cd,A	1.3	3.1	1.1	1.1
A-A10-3	11.7	8676.0	5244.1	—	3147.5	744.7^e,C	450.1^d,B	—	270.2^ef,A	2.6	7.3	—	2.0
A-A12-2	15.7	5998.3	3958.6	2900.1	4412.9	382.7^b,C	252.6^b,AB	185.1^b,A	281.6^ef,B	1.6	5.1	0.9	1.6
A-A12-3	23.4	8926.5	8070.1	5137.2	5478.8	381.5^b,C	344.9^c,B	219.6^bc,A	234.2^de,A	1.6	6.9	1.0	1.4
B-S8-2	14.4	7940.5	11206.2	9283.6	6890.3	551.3^d,B	778.1^g,D	644.5^f,C	478.4^g,A	1.5	5.6	2.4	1.3
B-S11-2	39.6	8940.0	11731.8	14721.4	10795.8	225.^5a,A	295.9^bc,A	371.3^e,B	272.3^ef,A	0.7	2.7	1.4	1.4
B-S11-6	14.6	6571.5	341.7	3373.0	290.0	451.3^bc,C	23.4^a,A	231.6^bc,B	19.9^a,A	1.5	0.2	0.9	0.9
B-A12-2	8.6	9355.1	9148.3	5661.7	4973.9	1090.1^f,B	1066^h,B	659.7^f,A	579.6^h,A	4.5	21.4	3.1	3.1
B-A17-1	17.6	9441.5	5148.7	4895.6	3027.3	537.2^d	292.9^bc	278.5^cd	172.2^c	2.4	2.4	1.8	1.8

The BLIS-gel filtration fraction that was not shown showed little or no antibacterial activity against the 4 tested bacteria. Description of BLIS-GF fraction code name: A/B (beginning of code): fraction with of 3-10 kDa (A) or <3 kDa (B) as a result of separation with an ultrafiltration membrane; S5/S8/S11/A10/A12/A17 (middle of code): BLIS-producing isolates; numbers 1-7 (end of code): fraction number assigned based on the results of BLIS separation by gel filtration; (EC) E. coli; (SA) S. aureus; (LM) L. monocytogenes; (ST) S. Typhimurium. The different small letters in the same rows showed a significant difference () between the BLIS-GF samples. The different capital letters in the same column show a significant difference () between test bacteria.