Purification, HR-LC-ESI-MS-MS Identification, and Peptide Prediction of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from Chanos chanos

Table 2

Toxicity (LC₅₀) analysis and stability analysis of selected BLIS-gel filtration fraction against high temperature and wide range of pH treatment.

BLIS-GF

Control (mm)

Inhibition zone (mm) after high-temperature treatment

Inhibition zone (mm) after wide-range pH treatment

LC50; (μg mL^-1)

60 °C

80 °C

100 °C

121 °C

2

4

6

7

8

10

A-S5-3

^E,E

^a,C

^e,D

^,B

^f,A

^c,C

^e,D

^hi,D

^g,D

^i,B

^,A

128.0^c

A-S8-2

^E,D

^h,D

^g,C

^j,B

^d,A

^c,B

^cd,B

^i,C

^g,C

^h,A

^i,A

93.9^a

A-S8-5

^D,F

^f,D

^e,C

^c,B

0^a,A

^ef,F

^ab,E

^a,D

^a,C

^a,B

0^a,A

139.9^d

A-S11-2

^E,DE

^d,D

^b,C

^a,B

0^a,A

^cde,D

^d,DE

^ghi,E

^e,C

^g,B

^h,A

258.1^i

A-S11-3

^E,D

^b,D

^c,C

^f,B

^b,A

^c,D

^e,E

^e,D

^d,C

^f,B

^g,A

549.4^m

A-S11-7

^D,E

^e,D

^a,C

^a,B

0^a,A

^a,A

^ab,C

^ef,E

^f,E

^j,D

^i,B

193.2^h

A-A10-3

^E,E

^f,D

^d,C

^b,B

0^a,A

^b,D

^e,E

^d,D

^a,C

^a,B

0^a,A

266.9^j

A-A12-2

^E,F

^b,D

^c,C

^f,B

^c,A

^cd,F

^d,F

^c,D

^b,C

^c,B

^b,A

707.5^n

A-A12-3

^D,E

^g,D

^f,C

^d,B

0^a,A

^g,E

^e,E

^c,D

^b,C

^d,B

^d,A

177.5^g

B-S8-2

^C,D

^g,E

^i,D

^j,B

^g,A

^c,D

^e,F

^fg,E

^e,C

^f,B

^e,A

151.3^e

B-S11-2

^D,E

^g,D

^h,C

^i,B

^c,A

^b,B

^d,C

^j,E

^g,D

^i,B

^h,A

316.5^l

B-S11-6

^D,E

^c,C

^b,B

^e,B

^b,A

^def,E

^c,E

^c,D

^b,C

^cd,B

^c,A

265.7^j

B-A12-2

^C,G

^h,C

^j,BC

^k,B

^e,A

^fg,E

^e,F

^b,D

^b,C

^b,B

0^a,A

100.3^b

B-A17-1

^D,F

^g,D

^e,C

^g,B

^e,A

^c,E

^a,D

^gh,F

^c,C

^e,B

^d,A

176.4^f

Description of BLIS-GF Fraction code name: A/B (beginning of code): fraction with of 3-10 kDa (A) or <3 kDa (B) as a result of separation with an ultrafiltration membrane; S5/S8/S11/A10/A12/A17 (middle of code): BLIS-producing isolates; numbers 1-7 (end of code): fraction number assigned based on the results of BLIS separation by gel filtration. (standard deviation) from three replications. The different small letters in the same rows showed significant differences () between the BLIS-GF samples; different capital letters in the same column showed significant differences () between treatments (pH or temperature).