International Journal of Food Science

Research Article

Purification, HR-LC-ESI-MS-MS Identification, and Peptide Prediction of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from Chanos chanos

Table 3

Peptide sequence prediction, in silico analysis of physicochemical properties and prediction antimicrobial peptide candidate.


BLIS-GF	Test bacteria	Peptide’s sequence	Net charge¹	Hydrophobicity¹ (kcal mol^-1)	Molecular mass (Da)²	Antimicrobial peptide prediction³

AS53	EC	FE	− 1	9.82	295.13	0.64
	SA	SLK	+1	9.91	347.23	0.86
	LM and ST	DPLD	− 2	14.07	459.21	0.64
AS82	EC	SWG	0	7.42	349.151	0.72
	SA	NFH	+1	9.37	417.19	0.64
	LM and ST	ELH	− 1	12.61	398.20	0.72
AS85	EC	WNR	+1	8.47	475.24	0.66
	SA	GVY	0	7.88	338.17	0.64
	LM and ST	DVV	− 1	10.62	331.17	0.64
BS82	EC	LD	− 1	10.29	247.13	0.65
	SA	RKLM	+2	10.59	546.78	0.72
	LM and ST	WK	+1	8.61	333.19	0.66
AS112	EC	PWKY	+1	8.04	593.31	0.68
	SA	DHTAY	− 1	13.91	606.25	0.55
	LM and ST	DWN	− 2	10.30	434.17	0.64
AS113	EC	GW	0	6.96	262.12	0.66
	SA	FH	+1	8.52	303.15	0.64
	LM and ST	LP	0	6.79	229.16	0.58
AS117	EC	DPQ	− 1	12.45	359.16	0.61
	SA	HCK	+1	13.01	387.18	0.69
	LM and ST	GPK	+1	11.99	301.19	0.69
BS112	EC	MP	0	7.37	247.11	0.64
	SA	NPKG	+1	12.84	415.23	0.55
	LM and ST	IFTEV	− 1	8.49	608.33	0.20
BS116	EC	MFHR	+1	9.66	590.29	0.64
	SA	EM	− 1	10.86	279.10	0.64
	LM and ST	SCF	+1	6.63	356.13	0.64
AA103	EC	IGNRR	+2	12.27	615.37	0.73
	SA	KFN	+1	9.84	408.22	0.59
	LM and ST	RKF	+2	10.80	450.28	0.75
AA122	EC	MKLAK	+2	12.08	590.37	0.55
	SA	SMW	0	5.60	423.17	0.62
	LM and ST	RI	+1	8.59	288.20	0.52
AA123	EC	SPF	0	6.79	350.17	0.58
	SA	DY	− 1	10.83	297.11	0.43
	LM & ST	CFP	− 1	6.31	366.148	0.67
BA122	EC	RM	+1	9.04	306.16	0.54
	SA	EMN	− 1	11.71	393.14	0.40
	LM and ST	HND	− 1	14.72	385.15	0.39
BA171	EC	EL	− 1	10.28	261.14	0.50
	SA	LDPL	− 1	9.18	457.27	0.57
	LM and ST	HVLTC	+1	8.75	572.29	0.78

Description of BLIS-GF fraction code name: A/B (beginning of code): fraction with of 3-10 kDa (A) or <3 kDa (B) as a result of separation with an ultrafiltration membrane; S5/S8/S11/A10/A12/A17 (middle of code): BLIS-producing isolates; numbers 1-7 (end of code): fraction number assigned based on the results of BLIS separation by gel filtration (EC) E. coli; (SA) S. aureus; (LM) L. monocytogenes; (ST) S. typhimurium. Bold letters indicate hydrophobic amino acid residues; prediction of physicochemical properties based on ¹PepDraw and ²Findpep; prediction of antimicrobial peptide candidate based on ³iAMPpep; net charge is the sum of positively (basic) and negatively (acidic) charge residues in neutral pH; molecular mass is the sum of monoisotopic masses of all amino acid residue in the peptide; hydrophobicity (Wimley-White scale) is the free energy associated with transitioning a peptide from an aqueous to hydrophobic environment. Antimicrobial peptide prediction shows possibility of peptide sequences that are predicted to be antimicrobial. A value >0.5 indicates a high probability as antimicrobial peptide.