New Information of the Anatomy and Phytochemical Screening of Pentaclethra macroloba (Willd.) Kuntze (Caesalpinioideae-Leguminosae) Seeds

Figure 5

Histochemical tests, positive reactions for different classes of metabolites present in the Pentaclethra macroloba (Willd.) Kuntze seed integument: (a) integument not submitted to tests (LM); (b) detection of total lipids with Sudan black (LM); (c) detection of total lipids with Sudan III (arrow) positive reaction for lipids in the cuticle (LM); (d) positive reaction for acid lipids (LM); (e) positive reaction for terpene oil resin in idioblasts (LM); (f) terpene reaction in idioblasts (LM); (g) positive reaction for total phenolic compounds; (h) positive reaction for lignin in the vascular bundle (LM); (i) positive reaction for tannin (LM); (j) positive reaction for pectin (LM); (k) positive reaction for pectin in the cuticle of the exotesta (arrowhead) and acidic mucilage () (LM); (l) positive reaction for neutral polysaccharides (LM); (m) starches (LM); (n) total proteins (LM); (o) idioblast with alkaloids (LM). Caption: al: alkaloids; am: starch; cf: phenolic compounds; li: lipids; tn: tannin; pn: total polysaccharides. Bar: 50 μm (d, l, n, h), 100 μm (b, c, e, f, g, h, i, o), 100 μm (a), and 200 μm (m).