New Information of the Anatomy and Phytochemical Screening of Pentaclethra macroloba (Willd.) Kuntze (Caesalpinioideae-Leguminosae) Seeds

Table 1

Histochemical tests applied to detect the main classes of metabolites.

Metabolic groups	Reagents	Reaction
Alkaloids	Dragendorff’s reagent [35]	Reddish brown
Starch	Lugol’s solution [28]	Black
Total phenolic compounds	Ferric chloride III SFF [28]	Black 1932
Calcium oxalate crystals	Hydrochloric acid [36]	Total dissociation of crystals
Lignin	Acid phloroglucinol [28]	Pink
Acid lipids	Nile blue A [37]	Blue
Total lipids	Sudan III [28]	Orange
	Sudan black [38]	Black
Acidic mucilage	Toluidine blue pH 4.7 [39]	Purple
	Ruthenium red [28]	Pink
Pectic compounds	Ruthenium red [28]	Pink
Total polysaccharides	Schiff test/periodic acid-PAS [40]	Pink
Total proteins	Xylidine ponceau [41]	Red
Tannin	Vanillin-hydrochloric acid [42]	Red
Terpene	Nadi reagent [43]	Blue (essential oil)
		Red (resin oil)
		Purple (mixture)