Research Article

mRNA/microRNA Profile at the Metamorphic Stage of Olive Flounder (Paralichthys olivaceus)

Table 2

Primers of miRNAs for stem-loop qRT-PCR.

microRNAPrimer namePrimer (5′-3′)

Universal revise primer5-GTGCAGGGTCCGAGGT
U6 snRNAU6RT5-GTCAGGCAGCGTGCAGGGTCCGAGGTATTCGCACGCTGCCTGACAAAAAT
U6F5-CGCAAGGATGACACGCAAATT
miR-11RT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACATAC
1F5-CGGCGGTGGAATGTAAAG
let-7a7aRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACTAT
7aF5-CGGCGGTGAGGTAGTAGGTT
let-7e & miR-7f7efRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACTAT
7efF5-CGGCGGTGAGGTAGTAGATT
let-7j7jRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACTGT
7jF5-CGGCGGTGAGGTAGTTGTTT
miR-9*9aRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTTTC
9aF5-CGGCGGTAAAGCTAGATAA
miR-10b10bRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAAAT
10bF5-CGGCGGTACCCTGTAGAACC
miR-20c20aRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATACCT
20aF5-CGGCGGTAAAGTGCTTATAGT
miR-2121RT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCAACA
21F5-CGGCGGTAGCTTATCAGACT
miR-23a23aRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGGAAA
23aF5-CGGCGGATCACATTGCCAGG
miR-23c23bRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACATGGTA
23bF5-CGGCGGATCACATTGCCAGGG
miR-26a26aRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGCCTA
26aF5-CGGCGGTTCAAGTAATCCAG
miR-125b125bRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACAAG
125bF5-CGGCGGTCCCTGAGACCCT
miR-128128RT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAGAG
128F5-CGGCGGTCACAGTGAACCG
miR-130d130cRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCCTTT
130cF5-CGGCGGCAGTGCAATAT
miR-145145RT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGGATT
145F5-CGGCGGGTCCAGTTTTCCCA
miR-181a181aRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTCAC
181aF5-CGGCGGAACATTCAACGCTGT
miR-181e181a-1RT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACTCA
181a-1F5-CGGCGGAACATTCAACGCTGTC
miR-181f181fRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCCCC
181fF5-CGGCGGAACATTCATTGCTGT
miR-200a200aRT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACATCG
200aF5-CGGCGGTAACACTGTCTGGT
miR-221221RT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAAACC
221F5-CGGCGGAGCTACATTGTCTGC
miR-429429RT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACGGCA
429F5-CGGCGGTAATACTGTCTGGT
miR-724724RT5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACAGT
724F5-CGGCGGTTAAAGGGAATTTG