Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense

Table 2

Primers used in our study.

Name	Sequence (5′-3′)
MnHc-s1	GTCGACTCTACTCCTCTTGG
MnHc-a1	TCGGTTATCCTTCAGCTC
MnHc-s2	TTCTGCTGATGCCTCCAA
MnHc-a2	TTCTTCACGGTGCCTGTC
MnHc-5′GSP	GGCGGTGAACAGCTTCTCCATTATC
MnHc-3′GSP	GCATGATGTGAACTTCCTCCTGTGG
5′-RACE CDS primer A	(T)25V N (N = A, C, G or T; V = A, G or C)
3′-RACE CDS primer A	AAGCAGTGGTATCAACGCAGAGTAC(T)30 V N
UPM	CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT
	CTAATACGACTCACTATAGGGC
β-Actin-s	GTGCCCATCTACGAGGGTTA
β-Actin-a	CGTCAGGGAGCTCGTAAGAC