Evidence of Divergent Amino Acid Usage in Comparative Analyses of R5- and X4-Associated HIV-1 Vpr Sequences

Figure 2

Position-specific Vpr amino acid diversity is generally consistent across multiple amino acid alphabets but different between R5- and X4-associated sequences. The amino acid diversity was calculated across all positions of Vpr for both the R5 (red) and X4 (blue) sequence populations. While the overall levels of variation tended to be consistent between the two populations, there were notable differences in amino acid diversity between the R5 and X4 populations that persisted across multiple alphabets at positions 28, 37, 55, and 89. Diversity values for amino acid positions at which at least one diversity value was greater than 2 in the full amino acid alphabet are listed in Table 2.