Development of a New Marker System for Identification of Spirodela polyrhiza and Landoltia punctata

Figure 2

Electropherograms showing capillary electrophoresis separation of the PCR product fragments amplified from ecotypes with the three primers. The horizontal axis displays the size of the detected PCR product fragments, while the vertical axis presents the intensity of the signal (i.e., the indicator of concentration of fragments in the PCR products). The orange peaks match the standard fragments in the GeneScan 500 LIZ size standard, while the blue ones represent the PCR products fragments amplified from different ecotypes. The numbers on the horizontal axis represent the size of the corresponding peak in the GeneScan 500 LIZ size standard (orange). “LH” represents Landoltia punctata haplotype number and “SH” represents Spirodela polyrhiza haplotype number. Different haplotypes displayed different types of blue peaks (DNA fragments) and combinations.