Differential MicroRNA Analyses of Burkholderia pseudomallei- and Francisella tularensis-Exposed hPBMCs Reveal Potential Biomarkers

Table 2

Summary table of the number of miRNAs differentially expressed in RNAseq, included in the qPCR assay, and confirmed in qPCR.

Sample	Number of miRNAs
	Differentially expressed in RNAseq			Included in the qPCR assay			Confirmed in qPCR
	Down	Up	Total	Down	Up	Total	Down	Up	Total
E. coli-exposed hPBMCs	21	13	34	3	0	3	0	0	0
B. pseudomallei-exposed hPBMCs	19	8	27	5	8	13	0	0	0
F. tularensis-exposed hPBMCs	12	14	26	3	15	18	1	0	1

In addition to the 14 miRNA species found to be upregulated, hsa-miR-146a-5p was also included in the qPCR assay as it is a common miRNA often found to be involved in response to bacterial infection.