In Vitro Susceptibility and Florfenicol Resistance in Citrobacter Isolates and Whole-Genome Analysis of Multidrug-Resistant Citrobacter freundii

Table 2

Primers used for the detection of florfenicol resistance genes in this work.

Target gene	Primer	Primer sequence (5-3)	Amplicon size (bp)	Reference
floR	floR-F	GGCTTTCGTCATTGCGTCTC	650	Zhang et al. [44]
	floR-R	ATCGGTAGGATGAAGGTGAGGA
fexA	fexA-F	GTACTTGTAGGTGCAATTACGGCTGA	1,272	Kehrenberg and Schwarz [45]
	fexA-R	CGCATCTGAGTAGGACATAGCGTC
fexB	fexB-F	TTCCCACTATTGGTGAAAGGAT	816	Liu et al. [23]
	fexB-R	GCAATTCCCTTTTATGGACGTT
pexA	pexA-F	ACAGTGCAGGTCGAAGAACC	215	Zhao et al. [46]
	pexA-R	TGCATTACCAATCGACATCC
optrA	optrA-F	AGGTGGTCAGCGAACTAA	1,395	Wang et al. [25]
	optrA-R	ATCAACTGTTCCCATTCA
Cfr	cfr-F	TGAAGTATAAAGCAGGTTGGGAGTCA	746	Kehrenberg and Schwarz [47]
	cfr-R	ACCATATAATTGACCACAAGCAGC
estDL136	estDL136-F	TGCCCGCACCCGATTTCT	864	Zhang et al. [48]
	estDL136-R	GATTGGATGCACCTCGTTCTA