In Vitro Susceptibility and Florfenicol Resistance in Citrobacter Isolates and Whole-Genome Analysis of Multidrug-Resistant Citrobacter freundii

Table 3

Primers used for functional detection of the resistance genes in this work.

Target gene	Primer	Sequence (5-3)^a	Restriction endonuclease	Vector	Annealing temperature (°C)	Amplicon size (bp)
floR	floR-F	GCTCTAGATTAGGGCGGCAGAGGGGGCTGGAAC	XbaI	pUCP24	64	1,477
	floR-R	CCAAGCTTTTAGACGACTGGCGACTTCTCGGTG	HindIII
catA2	catA2-F	CGGAATTCAATAAAACCGGGCTTAATACAGATT	EcoRI	pUCP20	58	808
	catA2-R	GCTCTAGATTATTTCAGTATGTTATCACACATC	XbaI
blaCMY-97	blaCMY-97-F	GCTCTAGATTATTATGGGTAGAAATATGCAAAT	XbaI	pUCP24	58	1,688
	blaCMY-97-R	CCAAGCTTTTATTGCAGTTTTTCAAGAATGCGC	HindIII
blaDHA-1	blaDHA-1-F	GCTCTAGATAATCCACCTGTAAGTTTTTCTTTA	XbaI	pUCP24	58	1,267
	blaDHA-1-R	CCAAGCTTTTATTCCAGTGCACTCAAAATAGCC	HindIII
blaSHV-12	blaSHV-12-F	GCTCTAGAATGGGTTCATGTGCAGCTCCATCAG	XbaI	pUCP24	58	1,023
	blaSHV-12-R	CCAAGCTTTTAGCGTTGCCAGTGCTCGATCAGC	HindIII
blaTEM-1b	blaTEM-1b-F	GCTCTAGAAGTATTGCCCGCTCCACGGTTTATA	XbaI	pUCP24	58	1,113
	blaTEM-1b-R	CCAAGCTTTTACCAATGCTTAATCAGTGAGGCA	HindIII
strA	strA-F	CGGAATTCCGGCCTGGTCCTTCAGCCACCATGC	EcoRI	pUCP20	58	1,590
	strA-R	GCTCTAGATCAACCCCAAGTCAGAGGGTCCAAT	XbaI
strB	strB-F	CGGAATTCGAACGAGAGCTACCGGTGCGGCTCG	EcoRI	pUCP20	58	1,167
	strB-R	GCTCTAGACTAGTATGACGTCTGTCGCACCTGC	XbaI
aac6	aac6-F	CGGAATTCAGGTTGCCGGGTGACGCACACCGTG	EcoRI	pUCP20	58	913
	aac6-R	GCTCTAGATTAGGCATCACTGCGTGTTCGCTCG	XbaI
aac3	aac3-F	CGGAATTCATCGCGATCCACGCTCAAACTGAAC	EcoRI	pUCP20	58	1,068
	aac3-R	GCTCTAGATCAGGGCGAGCCAAAGTGCCGTTGA	XbaI
aacc4cr	aacA4cr-F	CGGAATTCGGCGGTTTTCATGGCTTGTTATGAC	EcoRI	pUCP20	58	874
	aacA4cr-R	GCTCTAGATTAGGCATCACTGCGTGTTCGCTCG	XbaI
aac(6)-IIc	aac(6)-IIc-F	CGGAATTCACGCACACCGTGGAAACGGATGAAG	EcoRI	pUCP20	58	913
	aac(6)-IIc-R	GCTCTAGATCATGACCACTTCCCCTTGATTTTG	XbaI
qnrB4	qnrB4-F	GGGGTACCCCCTACCGCTGGATCTGCGTGAATT	KpnI	pUCP24	62	901
	qnrB4-R	CCAAGCTTTTAACCCATGACAGCGATACCAAGA	HindIII
qnrB6	qnrB6-F	CGGAATTCGCCAGCCTTTCATGATATATCTCCC	EcoRI	pUCP24	58	904
	qnrB6-R	CGGGATCCCTAACCAATCACCGCGATGCCAAGC	BamHI

^aThe underlined sequences represent the restriction endonuclease sites and their protective bases.