Florfenicol Resistance in Enterobacteriaceae and Whole-Genome Sequence Analysis of Florfenicol-Resistant Leclercia adecarboxylata Strain R25

Table 2

Primers used in this work.

Primer	Sequence (5-3)	Purpose	Product length (bp)	Annealing temperature (°C)
27F	AGAGTTTGATCCTGGCTCAG	16S rRNA	1465	55
1492R	TACGGCTACCTTGTTACGACTT
floR-F	ATGGTGATGCTCGGCGTGGGCCA	floR gene screening	800	58
floR-R	GCGCCGTTGGCGGTAACAGACACCGTGA
cfr-F	GGGAGGATTTAATAAATAATTTTGGAGAAACAG	cfr gene screening	580	58
cfr-R	CTTATATGTTCATCGAGTATATTCATTACCTCATC
pexA-F	CTTCCAGTTGAGAAGCGAGC	pexA gene screening	319	56
pexA-R	AGAAGCATACCCGTGAACATG
fexA-F	CTCTTCTGGACAGGCTGGAA	fexA gene screening	332	57
fexA-R	CCAGTTCCTGCTCCAAGGTA
fexB-F	ACTGGACAGGCAGGCTTAAT	fexB gene screening	319	57
fexB-R	CCTGCCCCAAGATACATTGC
optrA-F	CTTATGGATGGTGTGGCAGC	optrA gene screening	309	56
optrA-R	CCATGTGGTTTGTCGGTTCA
estDL136-F	ATGCCGTTAAACCCCCATGTCGAAG	estDL136 gene screening	933	55
estDL136-R	TCAAGCGAGGTCTCTTTTAAGATT
pro-aac(6)-Ib-cr-F	GCTATCAGGTCAAGTCTGCTTTTATT	aac(6)-Ib-cr gene cloning	674	62
pro-aac(6)-Ib-cr-R	TTAGGCATCACTGCGTGTTCGCTCG
pro-aadA16-F	GTGTTTCCATCTATAGAAGCAGCAATG	aadA16 gene cloning	1274	56
pro-aadA16-R	TTAAGCTGCGCCGCGAAGCGGCGTC
pro-arr-3-F	GGTGACCAACAGCAACGATTCCGTCAC	arr-3 gene cloning	1104	62
pro-arr-3-R	CTAGTCTTCAATGACGTGTAAACCAC
pro-qnrB6-F	GTTATTATGCACGGCTTACAGCAGGCAA	qnrB6 gene cloning	849	62
pro-qnrB6-R	CTAACCAATCACCGCGATGCCAAGCCG
pro-floR-F	GTTGCGAAGCAAAAGATAATCGGATAAA	floR gene cloning	1415	62
pro-floR-R	TTAGACGACTGGCGACTTCTCGGTGGCA