Differential Expression of miR-136 in Gestational Diabetes Mellitus Mediates the High-Glucose-Induced Trophoblast Cell Injury through Targeting E2F1

Figure 4

miR-136 directly regulates E2F1 in trophoblast cells and their negative correlation. (a) The putative binding site of miR-136 at the 3-UTR of E2F1. (b) The relative luciferase activity was inhibited by the overexpression but enhanced by the knockdown of miR-136 in wild-type (WT) 3UTR group (). (c, d) The expression of E2F1 was inhibited by high glucose, and this effect was strengthened by miR-136 overexpression, but was weakened by the silencing of miR-136 in both HRT-8/SVneo and BeWo cells ( vs. normal; ^### vs. HG). (e, f) The expression of E2F1 in serum and placental tissues was decreased in GDM patients compared with healthy controls (). (g, h) The levels of miR-136 were negatively correlated with E2F1 levels in GDM patients. HG: high glucose.

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