Research Article
circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8
Figure 3
miR-34a-5p was a target of circHUWE1. (a) The candidate miRNAs were enriched by Circinteractome, Starbase, and circBank. (b, c) The expression levels of candidate miRNAs were assessed by RT-qPCR in 549/DDP and H1299/DDP cells transfected with sh-circHUWE1 or sh-NC. (d) miR-34a-5p binding sites on circHUWE1 were presented. (e) miR-34a-5p level was examined by RT-qPCR in 549/DDP and H1299/DDP cells transfected with miR-34a-5p or miR-NC. (f–i) Dual-luciferase reporter assay and RIP assays were performed in 549/DDP and H1299/DDP cells. (j, k) The expression level of miR-34a-5p was examined by RT-qPCR in NSCLC tissues and cells. (l) The correlation between miR-34a-5p and circHUWE1 was analyzed by Pearson’s correlation analysis. .
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