Research Article
circHUWE1 Exerts an Oncogenic Role in Inducing DDP-Resistant NSCLC Progression Depending on the Regulation of miR-34a-5p/TNFAIP8
Figure 4
Knockdown of miR-34a-5p abolished circHUWE1-silencing-mediated effects on NSCLC cells. (a) RT-qPCR was employed to detect miR-34a-5p level in A549/DDP and H1299/DDP cells transfected with anti-miR-NC or anti-miR-34a-5p. (b–q) A549/DDP and H1299/DDP cells were transfected with sh-NC, sh-circHUWE1, sh-circHUWE1+anti-miR-NC, or sh-circHUWE1+anti-miR-34a-5p. (b) miR-34a-5p level was assessed by RT-qPCR. (c–f) CCK-8 assay was applied to test IC50 of DDP in A549/DDP and H1299/DDP cells. (g–i) The proliferation ability of A549/DDP and H1299/DDP cells was examined by colony forming and CCK-8 assays. (j–l) Flow cytometry was implemented to analyze cell apoptosis and cell cycle distribution. (m, n) Transwell assay was conducted in A549/DDP and H1299/DDP cells. (o–q) Western blot assay was used to assess levels of PCNA, c-caspase 3, and MMP 13. .
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