Genetic Diversity of Tef [Eragrostis tef (Zucc.)Trotter] as Revealed by Microsatellite Markers

Table 2

Locus name, expected size, annealing temperature, and sequence of the 10 selected microsatellite primers.

Primer	Expected size [bp]	Annealing T [°C]	Forward primer	Reverse primer
CNLTs2	160-260	58.2	CAGCAGGGAGAGAGAGGAGA	GGGGTCAAGTTATTGCTTAGAGAA
CNLTs5	190-310	57.2	CCCAAAGTGATGCAAAAACA	TAGATAGAGACACAGACACACACA
CNLTs6	90-260	58.2	AATTCGCAGCTGATCTACGC	CTCGTCGATATACGTGCAAAA
CNLTs19	180-300	58.2	CATTTCTTGCTGCTGGATCA	AGTATGGTGGCCTTGGTGAG
CNLTs22	100-260	55.7	CATGCTCGTTCAGAGTCCAA	GGGGGATCTAGGAGAGAGAGA
CNLTs24	135-180	55.7	GAGAGCGGTTTTGTCCTACG	GGAATAGGGAGGCGAGGTAG
CNLTs25	150-315	57.2	TTGGAATGAGATGGCATTTG	GAAGCGGGGTAAGATTTGAA
CNLTs458	130-250	59.1	AACAAGAACCACACAACA	AAAGGAACCCACAGGGGTAA
CNLTs461	220-320	63.9	GTCTTGATGGTGGCGGAATAG	TCATCATCCTGCTCGAATCA
CNLTs463	190-260	59.1	TGCTAGGATGGTCCTGTTGAG	AGCACCAAATCCCTATGCAC