ALDH1A3–Linc00284 Axis Mediates the Invasion of Colorectal Cancer by Targeting TGFβ Signaling via Sponging miR-361-5p

Figure 4

ALDH1A3–Linc00284 axis promoted EMT via regulating TGFβ level. The mRNA level of E-cadherin (a); N-cadherin (b); and vimentin (c) in Linc00284- and ALDH1A3-silenced SW480 cells was determined by qPCR analysis. The protein level of E-cadherin ((d) and (e)), N-cadherin ((d) and (f)), and vimentin ((d) and (g)) in Linc00284- and ALDH1A3-silenced SW480 cells was determined by western blot analysis. (h) The mRNA level of TGFβ in Linc00284- and ALDH1A3-silenced SW480 cells was determined by qPCR analysis. (i) The protein level of TGFβ in Linc00284- and ALDH1A3-silenced SW480 cells medium was determined by ELISA. The mRNA level of E-cadherin (j); N-cadherin (k), and vimentin (l) in Linc00284- and ALDH1A3-overexpressed SW480 cells was determined by qPCR analysis. The protein level of E-Cadherin ((m) and (n)), N-Cadherin ((m) and (o)), and Vimentin ((m) and (p)) in Linc00284- and ALDH1A3-overexpressed SW480 cells was determined by western blot analysis. (q) The mRNA level of TGFβ in Linc00284- and ALDH1A3-overexpressed SW480 cells was determined by qPCR analysis. (r) The protein level of TGFβ in Linc00284- and ALDH1A3-overexpressed SW480 cells medium was determined by ELISA. Overexpression of Linc00284 rescued the effect of ALDH1A3 silence on TGFβ mRNA level in SW480 cell (s) and protein level (t) in medium (3 wells per group).

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