ALDH1A3–Linc00284 Axis Mediates the Invasion of Colorectal Cancer by Targeting TGFβ Signaling via Sponging miR-361-5p

Figure 5

ALDH1A3–Linc00284 axis mediated the expression of TGFβ by sponging miR-361-5p. (a) Schematic diagram of the potential binding sites between Linc00284 and miR-361-5p. Dual-luciferase reporter assay (b) and RIP experiment (c) and (d) confirmed the interaction between Linc00284 and miR-361-5p. (e) and (f) The expression level of miR-361-5p in ALDH1A3- or Linc00284-silenced (e) and -overexpressed (f) SW480 cells. (g) Schematic diagram of the potential binding sites between 3'-UTR of TGFβ mRNA and miR-361-5p. (h) Dual-luciferase reporter assay confirmed the interaction between TGFβ mRNA and miR-361-5p. The mRNA level in cells (i) and protein in medium (j) of TGFβ in miR-361-5p mimics- or inhibitors-treated SW480 cells were examined by qPCR and ELISA, respectively. The mRNA level of E-Cadherin (k), N-cadherin (l), and Vimentin (m) in miR-361-5p mimics- or inhibitors-treated SW480 cells was examined by qPCR.

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