The Novel Action of miR-193b-3p/CDK1 Signaling in HCC Proliferation and Migration: A Study Based on Bioinformatic Analysis and Experimental Investigation

<div>miR-193b-3p negatively modulated CDK1. (a) miRNA enrichment analysis of DEGs. (b) The predicted binding location between miR-193b-3p and CDK1 3′UTR. (c)–(e) The HEK293T cells were transfected with mimics NC or miR-193b-3p mimics, and relative expression of miR-193b-3p in HEK293T cells was measured by qRT-PCR; (d) relative luciferase activity of wild-type (WT) CDK1 3′UTR reporter vector in HEK293T cells was evaluated by Dual-Luciferase reporter assay kit; (e) the relative luciferase activity of mutant (MUT) CDK1 3′UTR reporter vector in HEK293T cells was determined by the Dual-Luciferase reporter assay kit; and (f) mRNA expression level of CDK1 in the SMMC7721 cells. <span class="nowrap"><svg height="8.8423pt" id="M16" style="vertical-align:-0.2064009pt" version="1.1" viewbox="-0.0498162 -8.6359 32.2343 8.8423" width="32.2343pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M822 650H589L583 622C660 617 677 607 674 561C672 534 664 481 647 390L600 137H596L273 650H126L120 622C176 620 194 615 207 594C221 571 225 557 214 504L161 257C141 166 129 112 121 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International Journal of Genomics

fig10

Figure 10

Figure 10: The Novel Action of miR-193b-3p/CDK1 Signaling in HCC Proliferation and Migration: A Study Based on Bioinformatic Analysis and Experimental Investigation 

Figure 10 | The Novel Action of miR-193b-3p/CDK1 Signaling in HCC Proliferation and Migration: A Study Based on Bioinformatic Analysis and Experimental Investigation 