circ_0008285 Regulates Glioma Progression via the miR-384/HMGB1 Axis

Figure 3

circ_0008285 regulates the expression of HMGB1 protein by targeting miR-384. (a) Circinteractome database prediction of the binding sequence of circ_0008285 and miR-384. (b) Dual luciferase assay using WT-Circ or MUT-Circ vector in the presence or absence of miR384 mimic. (c) miR-384 expression level after circ_0008285 silencing was detected by qRT-PCR. (d) StarBase prediction of miR-384 complementary site at HMGB1 mRNA 3 UTR. (e) Dual luciferase assay using WT-HMGB1 or MUT-HMGB1 vector in the presence or absence of miR384 mimic. (f) Western blot analysis of HMGB1 expression after miR-384 overexpression. (g) qRT-PCR analysis of HMGB1 and miR-384 in glioma tumor samples was higher than in normal brain tissues. (h) Correlation analysis of circ_0008285, miR-384, and HMGB1 in glioma tumor specimens. ***.

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