Hsa_circ_0003220 Drives Chemoresistance of Human NSCLC Cells by Modulating miR-489-3p/IGF1

Figure 3

Hsa_circ_0003220 served as miR-489-3p’s sponge. (a)The potential binding sites between hsa_circ_0003220 and miR-489-3p. (b) A DLR experiment to find out if hsa_circ_0003220 and miR-489-3p interacted in 293T cells. (c) RIP assay was performed to confirm whether hsa_circ_0003220 is bound to miR-489-3p. (d) qRT-PCR assay was employed to ascertain the miR-489-3p level in H460, A549, H460/PTX, and A549/PTX cells. (e) qRT-PCR analysis was employed to determine whether or not miR-489-3p expression was increased in H460/PTX and A549/PTX cells transfected with si-hsa_circ_0003220 or si-NC. (f) miR-489-3p expression was evaluated by qRT-PCR in both PS and PR NSCLC samples. (g) Spearman’s correlation coefficient analysis estimated the correlation between miR-489-3p and hsa_circ_0003220 in PR tumor tissues. (h) miR-489-3p expression was quantified in anti-miR-NC and anti-miR-489-3p transfected H460/PTX and A549/PTX cells using a qRT-PCR technique. (i) The MTT assay was used to determine cellular proliferation. (j) The MTT test was used to calculate the IC50 values of DTX, DDP, and PTX. *.

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