Hsa_circ_0003220 Drives Chemoresistance of Human NSCLC Cells by Modulating miR-489-3p/IGF1

Figure 4

IGF1, a direct miR-489-3p target gene. (a) MiR-489-3p and IGF1 3-untranslated region (UTR) binding sites, predicted computationally. (b) DLR assay to examine the cooperation between IGF1 and miR-489-3p in 293T cells. (c–f) A combination of qRT-PCR and ELISA was used to quantify IGF1 expression. (g) IGF1 levels in PR and PS NSCLC tissues were analyzed using qRT-PCR. (h) Spearman’s correlation coefficient analysis estimated the correlation between IGF1 and miR-489-3p in PR tumor tissues. *.

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