Stratifin Promotes Hepatocellular Carcinoma Progression by Modulating the Wnt/β-Catenin Pathway

Figure 3

SFN promotes HCC cell proliferation, migration, and invasion in vitro and tumor growth in vivo. The efficiency of overexpression or knockdown of SFN was determined by RT–qPCR and WB in SMMC-7721 (a and b) and MHCC-97H (c and d) cell lines. Wound healing assays (e and f) and transwell migration assays (g and h) were used to explore the migratory ability of HCC cells with upregulated or downregulated SFN expression. (g and h) The invasion capacity of HCC cells with upregulated or downregulated SFN expression was examined by transwell invasion assays. (i and j) Colony formation assays were utilized to investigate the proliferation ability of HCC cells with altered SFN expression. (k and l) CCK-8 assays were used to evaluate the proliferation capacity of MHCC-97H cells with SFN knockdown and SMMC-7721 cells with SFN overexpression. (m) Xenograft mouse models were used to analyze the role of SFN in tumor growth. Images of subcutaneous tumors derived from MHCC-97H cells with SFN knockdown or SMMC-7721 cells with SFN overexpression in nude mice. (n and o) The weight and volume of the tumors in each xenografted mouse in the model. *, **, ***, and ****. All data are from three independent experiments.

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