Stratifin Promotes Hepatocellular Carcinoma Progression by Modulating the Wnt/β-Catenin Pathway

Figure 4

SFN induces EMT and activates Wnt/β-catenin signaling in HCC cells. (a) The protein levels of E-cadherin, N-cadherin, vimentin, Snail, MMP2, and MMP9 in MHCC-97H cells infected with lentiviruses expressing shSFN#1, shSFN#2, and shNC, as determined by WB. (b) The protein levels of E-cadherin, N-cadherin, vimentin, Snail, MMP2, and MMP9 in SMMC-7721 cells infected with lentiviruses carrying the SFN overexpression plasmid or Vector, as determined by WB. (c) The protein levels of β-catenin, non-phospho-β-catenin (active), GSK-3β, phospho-GSK-3β (Ser9), c-Myc, and Axin2 in MHCC-97H cells infected with lentiviruses expressing shSFN#1, shSFN#2, or shNC, as determined by WB. (d) The protein levels of β-catenin, non-phospho β-catenin (active), GSK-3β, phospho-GSK-3β (Ser9), c-Myc, and Axin2 in SMMC-7721 cells infected with lentiviruses carrying the SFN overexpression plasmid or Vector, as determined by WB. (e) The protein level of β-catenin in the nucleus of MHCC-97H cells infected with lentiviruses expressing shSFN#1, shSFN#2, or shNC, as determined by WB. (f) The protein levels of β-catenin in the nucleus of SMMC-7721 cells infected with lentiviruses carrying the SFN overexpression plasmid or Vector, as determined by WB. (g) TOP/FOP luciferase reporter activity in MHCC-97H cells with SFN knockdown. (h) TOP/FOP luciferase reporter activity in SMMC-7721 cells overexpressing SFN. All data are from three independent experiments.

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