Recovery of the Cell Cycle Inhibition in -Induced Cirrhosis by the Adenosine Derivative IFC-305
Figure 2
Effect of IFC305 treatment on cyclin D1, cyclin E, cyclin A, and cyclin B1 protein expression in CCl4-induced cirrhosis in rats. (a), (c), (d), and (e) Expression of the indicated proteins from liver nuclear extracts was determined by western blot analysis. A representative western blot image of each one is shown. The bar graph represents the densitometry analysis expressed as arbitrary units of the mean ± SEM from 3 rats/group, values were normalized to β-actin immunodetection (image, Figure 1(a)). *Statistical difference () when compared to C group. #Statistical difference () when compared to their respective experimental group SS5 or SS10. (b) Effect of IFC305 treatment on cyclin D1 mRNA expression in CCl4-induced cirrhosis in rats. RNA was isolated from liver and mRNA expression for cyclin D1 was analyzed by quantitative RT-PCR as described in Section 2. Arbitrary units were normalized with Arbp mRNA gene expression level. Data represent mean ± SEM from 3 rats/group. *Statistical difference () compared to C group. #Statistical difference () when compared to their respective experimental group SS5 or SS10.
