Recovery of the Cell Cycle Inhibition in -Induced Cirrhosis by the Adenosine Derivative IFC-305
Figure 4
Effect of IFC305 treatment on phospho-Rb (Ser 795), E2F1, and DP1 proteins expression in cirrhotic livers. (a), (c), and (d) Expression of the indicated proteins from liver nuclear extracts was done by western blot analysis. A representative western blot image of each one is shown. The bar graph represents the densitometry analysis expressed as arbitrary units of the mean ± SEM from 3 rats/group; values were normalized to β-actin immunodetection (image, Figure 1(a)). *Statistical difference () when compared to C group. #Statistical difference () when compared to their respective experimental group SS5 or SS10. (b) Effect of IFC305 treatment on Rb mRNA expression in CCl4-induced cirrhosis in rats. RNA was isolated from liver and mRNA expression for Rb was analyzed by quantitative RT-PCR as described in Section 2. Arbitrary units were normalized with Arbp mRNA gene expression level. Data represent mean ± SEM from 3 rats/group. *Statistical difference () compared to C group. #Statistical difference () when compared to their respective experimental group SS5 or SS10.
