Recovery of the Cell Cycle Inhibition in -Induced Cirrhosis by the Adenosine Derivative IFC-305
Figure 5
Effect of IFC305 treatment on p53 and MDM2 proteins expression in cirrhotic livers. (a) and (c) Expression of the indicated proteins from liver nuclear extracts was done by western blot analysis. A representative western blot image of each one is shown. The bar graph represents the densitometry analysis expressed as arbitrary units of the mean ± SEM from 3 rats/group; values were normalized to β-actin immunodetection (image, Figure 1(a)). *Statistical difference () when compared to C group. #Statistical difference () when compared to their respective experimental group SS5 or SS10. (b) Effect of IFC305 treatment on p53 mRNA expression in CCl4-induced cirrhosis in rats. RNA was isolated from liver and mRNA expression for Rb was analyzed by quantitative RT-PCR as described in Section 2. Arbitrary units were normalized with Arbp mRNA gene expression level. Data represent mean ± SEM from 3 rats/group. *Statistical difference () when compared to C group. #Statistical difference () when compared to their respective experimental group SS5 or SS10.
