HMGB1 Aggravates Pressure Overload-Induced Left Ventricular Dysfunction by Promoting Myocardial Fibrosis

Table 1

Primer sequences for quantitative real-time PCR.

Gene	Forward (F) and reverse (R) primers
BNP	F 5′ CCTGGCCCATCGCTTCT3′
Collagen type I	R 5′ CATCTGGGACAGCACCTTCA3′
	F 5′ CTTCACCTACAGCACCCTTGTG3′
TGF-β1	R 5′ TGACTGTCTTGCCCCAAGTTC3′
	F 5′ GCAGTGGCTGAACCAAGGA3′
MMP2	R 5′ AGCAGTGAGCGCTGAATCG3′
	F 5′ GGACCCCGGTTTCCCTAA3′
β-Actin	R 5′ CAGGTTATCAGGGATGGCATTC3′
	F 5′ CGATGCCCTGAGGCTCTTT3′
	R 5′ TGGATGCCACAGGATTCCA3′

BNP: brain natriuretic peptide; TGF-β1: transforming growth factor-β1; MMP2: matrix metalloproteinase 2.