Screening of Kampo extracts and identification of major components that exhibit the inhibitory effects of IL-17 secretion. HJG, HST, OGT, SST, HET, JTT, DKT, or SRT were added at a concentration of 10 μg/mL to the two-way MLR of splenic lymphocytes obtained from BALB/c and SJL mice and then were incubated for seven days (a). The supernatants were analyzed using ELISA to detect the typical cytokine secretions of IFN-γ (center), IL-5 (right), and IL-17 (left) for Th1, Th2, and Th17, respectively. Increasing concentrations of wogonin or berberine (0.1, 0.3, and 1 μM) were mixed with a culture of lymphocytes obtained from BALB/c and SJL mice and then incubated for seven days (b). The supernatants were analyzed using ELISA to detect the typical cytokines of IFN-γ (center), IL-4 (right), and IL-17 (left) for Th1, Th2, and Th17, respectively.
