The Effect of Lithium on Inflammation-Associated Genes in Lipopolysaccharide-Activated Raw 264.7 Macrophages

Figure 6

Effect of lithium on nitric oxide (NO) production assessed using Griess reagent on LPS-activated Raw 264.7 cells. Cells were seeded at a density of 6 × 10⁶ cells/ml and treatment was executed by pretreating with 10 mM NaCl and lithium (1.25, 2.5, 5, and 10 mM) for an hour thereafter; other set of wells that are already treated with lithium and NaCl were stimulated with 10 μg/ml LPS for 24 hrs. Quantitation of NO production was carried out by GloMax-Multi microplate at 550 nm (Promega, USA). Treatments that encompass various concentrations of lithium and 10 mM NaCl combined with LPS were compared with LPS activated wells. Graphs were executed using GraphPad Prism 4 software and the statistical analysis was carried out using GraphPad InStat software and ANOVA (Tukey–Kramer) (; ).