Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis

Table 1

Characteristics of primers and amplicons.

Gene	Primer name	Sequence 5′ ⟶ 3′	Amplicon size [bp]	Amplicon location with reference to assembly GRCh38 [chromosome : start : end : strand]	Primer complementary to methylated/unmethylated sequences
ACT-B	ACTB sense	GGTGGTGATGGAGGAGGTTTAG	115	7 : 5532117 : 5532232 : −1	Independent of methylation status
	ACTB antisense	CCCTTAAAAATTACAAAAACCACAACC
SOCS3	SOCS3_ methyl_sense	GTGGAACGATGGTTTTAATTTACG	126	17 : 78360912 : 78361038 : 1	Methylated sequences
	SOCS3_ methyl_antisense	ATTCCCGCAAATCCCTAACG
	SOCS3_ unmethyl_sense	GGTGGAATGATGGTTTTAATTTATG	127	17 : 78360911 : 78361038 : 1	Unmethylated sequences
	SOCS3_ unmethyl_antisense	ATTCCCACAAATCCCTAACATAC
IL6R	IL6R_ methyl_sense	CGTATTTTGGGACGGTTTAGAGAC	91	1 : 154405299 : 154405390 : 1	Methylated sequences
	IL6R_ methyl_antisense	CACATAACTCAAAACGACGAACG
	IL6R_ unmethyl_sense	TTGTATTTTGGGATGGTTTAGAGATG	95	1 : 154405298 : 154405393 : 1	Unmethylated sequences
	IL6R_ unmethyl_antisense	TCACACATAACTCAAAACAACAAACAAT

CpG sites in primer sequence are in bold.