Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells
Table 2
Flow cytometry analysis of lymphocyte populations from the spleen of AIR mice (n = 5–8). The results are expressed as a means percentage and standard deviation of 10,000 events acquired from 107 cells/mL suspension.
Lymphocyte subsets
Days
Melanoma
B16F10
S91
Control
AIRmax
AIRmin
AIRmax
AIRmin
AIRmax
AIRmin
7
n = 7
n = 5
n = 5
n = 5
n = 6
n = 5
CD3+
30.62 ± 5.64
22.83 ± 2.18
24.66 ± 6.23
27.57 ± 5.21
24.84 ± 4.13
28.29 ± 1.77
CD4+
20.60 ± 4.39
14.41 ± 0.90
15.76 ± 0.87
17.16 ± 2.91
14.96 ± 1.61
17.57 ± 1.66
CD8+
15.13 ± 1.66
12.11 ± 3.98
10.47 ± 1.85
11.40 ± 1.72
16.20 ± 3.20
9.53 ± 4.69
CD4+/CD25+
3.92 ± 0.58A
2.89 ± 0.57B
1.48 ± 0.21B
2.94 ± 0.66A
2.87 ± 0.90
1.99 ± 1.35
CD3+/CD49b+
2.60 ± 0.40
2.01 ± 0.23
1.21 ± 0.62
1.62 ± 0.23
2.02 ± 0.56
1.77 ± 0.26
CD49b+
6.29 ± 0.84
5.90 ± 1.54
3.85 ± 0.85
4.18 ± 1.45
6.01 ± 1.14
6.30 ± 1.90
14
n = 8
n = 5
n = 8
n = 5
n = 8
n = 5
CD3+
32.47 ± 4.65
22.24 ± 2.54
36.86 ± 9.32
27.64 ± 4.56
39.55 ± 5.46A
25.89 ± 4.28B
CD4+
20.25 ± 2.58
15.04 ± 1.40
23.13 ± 4.96
18.23 ± 3.58
23.58 ± 2.92
16.76 ± 4.48
CD8+
12.46 ± 3.34
6.72 ± 1.17
14.38 ± 4.24A
8.07 ± 0.90B
15.20 ± 2.22A
9.04 ± 2.24B
CD4+/CD25+
2.64 ± 1.14A
0.87 ± 0.38B
2.64 ± 0.91
2.21 ± 1.29
2.90 ± 1.00
1.38 ± 0.86
CD3+/CD49b+
3.67 ± 0.99
3.23 ± 0.94
4.01 ± 0.87
3.22 ± 0.98
3.90 ± 1.22
3.94 ± 0.91
CD49b+
3.31 ± 0.51
4.61 ± 1.19
4.12 ± 0.85
4.58 ± 1.71
3.51 ± 1.01
3.98 ± 1.29
30
n = 5
n = 5
n = 5
n = 5
n = 5
n = 5
CD3+
30.69 ± 4.71a
24.12 ± 3.20
18.62 ± 4.95b
22.86 ± 2.45
32.94 ± 2.45a
23.66 ± 2.43
CD4+
19.65 ± 1.56a
17.29 ± 4.47
12.00 ± 2.97b
15.18 ± 1.27
22.07 ± 4.47a
16.82 ± 1.70
CD8+
11.91 ± 2.97a
8.84 ± 1.66
6.79 ± 1.96b
7.93 ± 2.00
10.62 ± 2.29
7.06 ± 2.22
CD4+/CD25+
2.60 ± 0.65
1.92 ± 0.75
1.42 ± 0.48
1.49 ± 0.49
2.51 ± 0.76
1.24 ± 0.69
CD3+/CD49b+
2.11 ± 0.83
1.33 ± 0.06
1.39 ± 0.88
1.26 ± 0.21
1.34 ± 0.19
1.30 ± 0.47
CD49b+
4.86 ± 0.65
3.79 ± 0.73
4.25 ± 0.86
4.28 ± 0.40
3.91 ± 0.26
3.92 ± 0.72
Lowercase letters indicate the comparison of lymphocyte subsets within the same AIR mouse line (a > b, < 0.05). Capital letters indicate a comparison of lymphocyte subsets between AIRmax and AIRmin (A > B, < 0.05).