In Vitro and In Vivo Anti-Inflammatory Properties of the Hydroethanolic Extract of the Roots of Vernonia guineensis (Asteraceae)

Table 3

General appearance and behavioral observations of acute toxicity study after hydroethanolic extract of V. guineensis administration.

Observation	After 4 hours				After 7 days
	Distilled water	250 mg/kg	2500 mg/kg	5000 mg/kg	250 mg/kg	2500 mg/kg	5000 mg/kg
Alertness	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Body weight	Normal	Not change	Not change	Not change	Not change	Not change	Not change
Grooming	Absent	Absent	Absent	Absent	Absent	Absent	Absent
Temperature	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Restlessness	Absent	Absent	Absent	Absent	Absent	Absent	Absent
Touch response	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Food intake	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Pain response	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Urination	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Righting reflux	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Pinna reflex	Present	Present	Present	Present	Present	Present	Present
Writhing	Absent	Absent	Absent	Absent	Absent	Absent	Absent
Tremors	Absent	Absent	Absent	Absent	Absent	Absent	Absent
Salivation	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Convulsion	Absent	Absent	Absent	Absent	Absent	Absent	Absent
Corneal reflex	Present	Present	Present	Present	Present	Present	Present
Lacrimation	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Water intake	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Rate of respiration	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Gripping	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Skin color	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Drowsiness	Not present	Not present	Not present	Present	Not present	Not present	Not present
Pupils	Normal	Normal	Normal	Normal	Normal	Normal	Normal
Sedation	No effect	No effect	No effect	Observed	No effect	No effect	No effect
Eye color	No effect	No effect	No effect	No effect	No effect	No effect	No effect
Diarrhea	Not present	Not present	Not present	Not present	Not present	Not present	Not present
General physique	Normal	Normal	Normal	Lethargy	Normal	Normal	Normal
Mortality	Nil	Nil	Nil	Nil	Nil	Nil	Nil