Comparison of Four Polymerase Chain Reaction Methods for the Rapid Detection of Human Fecal Pollution in Marine and Inland Waters

Table 1

Oligonucleotide primers (16S rDNA) used to detect fecal bacteria for each PCR assay.

Primer name and sequence (5^′-3^′)	Annealing temp (^∘C)	Product size (bp)	Method
lm26 (GATTCTGGCTCAGGATGAACG)	55	1350	Bonjoch et al. [12]
lm3 (CGGGTGCTICCCCACTTTCATG)
lm 26 (GATTCTGGCTCAGGATGAACG)	48	777	King et al. [10]
785R (CTACCAGGGTATCTAATCC)
BiADO1 (CTCCAGTTGGATGCATGTC)	55	279	Matsuki [16]
BiADO2 (CGAAGGCTTGCTCCCAGT)			Bonjoch et al. [12]
			King et al. [10]
HuBac566f (GGGTTTAAAGGGAGCGTAGG)	60	116	Layton et al. [7]
HuBac692r (CTACACCACGAATCCGCCT)

∗Primer pairs lm26-lm3 and lm26-785R are genus-specific primers for Bifidobacteria.
Primer pair BiADO1 and BiADO2 is species-specific for B. adolescentis
HuBAC566f and HUBAC692r are specific for human associated Bacteroides