Comparison of Four Polymerase Chain Reaction Methods for the Rapid Detection of Human Fecal Pollution in Marine and Inland Waters

Table 2

DNA recovered and qPCR estimation of Bifidobacterium adolescentis in sewage sample DNA extracts from 0.22 𝜇m and 0.45 𝜇m of filters.

Sample (𝑛=3)	DNA extracted (avg. ng/𝜇L)		qPCR of B. adolescentis (avg. ng) ×10^−2
	0.22 𝜇m	0.45 𝜇m	0.22 𝜇m	0.45 𝜇m
Sewage dilution 10^−1	14.00±1.66	16.17±1.30	2.418±0.23	0.142±0.12
Sewage dilution 10^−2	17.50±4.50	12.13±0.92	1.154±0.13	0.112±0.09
Sewage dilution 10^−3	17.83±3.10	14.57±3.32	0.856±0.25	0.288±0.08
Sewage dilution 10^−4	6.17±0.90	13.37±1.35	0.738±0.34	0.319±0.11