Comparison of Four Polymerase Chain Reaction Methods for the Rapid Detection of Human Fecal Pollution in Marine and Inland Waters

Table 3

Detection of human fecal bacteria (B. adolescentis and HuBAC) PCR Products in environmental samples by multiplex- [10, 12] and single-PCR assays.

Sample (𝑛)	Matsuki et al. [16]	Bonjoch et al. [12]	King et al. [10]	Layton et al. [7]
Raw sewage (3)	2	2	3	3
Sewage dilution 10^−2 on; .22 𝜇m filter (3)	0	2	3	2^∗
Sewage dilution 10^−3 on; .22 𝜇m filter (3)	0	1	3	0
Sewage dilution 10^−4 on; .22 𝜇m filter (3)	0	0	3	0
Sewage dilution 10^−1 on; .45 𝜇m filter (3)	0	3	3	0
Sewage dilution 10^−2 on; .45 𝜇m filter (3)	0	3	3	0
Sewage dilution 10^−3 on; .45 𝜇m filter (3)	0	1	3	0
Sewage dilution 10^−4 on; .45 𝜇m filter (3)	0	1	3	0
Pig (8)	0	3	0	6
Chicken (4)	0	0	0	0
Cow (2)	0	0	0	2^∗
Rabbit (4)	0	0	0	0
Horse (4)	0	0	0	1^∗

Detection limit was reached in 1/10,000 diluents of sewage.
∗Very faint bands