Research Article
Production of β-Glucosidase from a Newly Isolated Aspergillus Species Using Response Surface Methodology
Table 2
Treatment combinations and mean response for β-glucosidase production by A. niger SOI017.
| | Cellobiose (%) | Yeast extract (%) | (NH4)2SO4 (%) | pH | Experimental Sp activity (μmol/ml/mg) |
| 1 | 1.125 | 0.275 | 1.1 | 4.5 | 54.5 ± 6.3 | 2 | 1.125 | 0.275 | 1.1 | 7.5 | 6.6 ± 0.4 | 3 | 1.125 | 0.275 | 2.1 | 4.5 | 3927.0 ± 661.9 | 4 | 1.125 | 0.275 | 2.1 | 7.5 | 21.4 ± 5.3 | 5 | 1.125 | 0.725 | 1.1 | 4.5 | 3186.9 ± 68.3 | 6 | 1.125 | 0.725 | 1.1 | 7.5 | 1988.7 ± 707.2 | 7 | 1.125 | 0.725 | 2.1 | 4.5 | 2024.1 ± 263.2 | 8 | 1.125 | 0.725 | 2.1 | 7.5 | 908.4 ± 121.3 | 9 | 2.375 | 0.275 | 1.1 | 4.5 | 3.2 ± 0.1 | 10 | 2.375 | 0.275 | 1.1 | 7.5 | 4.5 ± 0.2 | 11 | 2.375 | 0.275 | 2.1 | 4.5 | 2.4 ± 0.1 | 12 | 2.375 | 0.275 | 2.1 | 7.5 | 4.5 ± 0.2 | 13 | 2.375 | 0.725 | 1.1 | 4.5 | 402.1 ± 32.4 | 14 | 2.375 | 0.725 | 1.1 | 7.5 | 146.2 ± 107.2 | 15 | 2.375 | 0.725 | 2.1 | 4.5 | 1378.6 ± 315.2 | 16 | 2.375 | 0.725 | 2.1 | 7.5 | 319.3 ± 31.6 | 17 | 3.000 | 0.500 | 1.6 | 6.0 | 445.2 ± 15.0 | 18 | 0.500 | 0.500 | 1.6 | 6.0 | 921.7 ± 93.3 | 19 | 1.750 | 0.950 | 1.6 | 6.0 | 1498.4 ± 122.7 | 20 | 1.750 | 0.050 | 1.6 | 6.0 | 3.2 ± 0.3 | 21 | 1.750 | 0.500 | 2.6 | 6.0 | 1467.9 ± 231.0 | 22 | 1.750 | 0.500 | 0.6 | 6.0 | 2677.1 ± 111.1 | 23 | 1.750 | 0.500 | 1.6 | 9.0 | 442.2 ± 18.3 | 24 | 1.750 | 0.500 | 1.6 | 3.0 | 4752.8 ± 139.2 | 25 | 1.750 | 0.500 | 1.6 | 6.0 | 1964.2 ± 265.1 | 26 | 1.750 | 0.500 | 1.6 | 6.0 | 2387.5 ± 252.1 | 27 | 1.750 | 0.500 | 1.6 | 6.0 | 2056.5 ± 33.4 | 28 | 1.750 | 0.500 | 1.6 | 6.0 | 3553.6 ± 288.4 | 29 | 1.750 | 0.500 | 1.6 | 6.0 | 1813.1 ± 114.5 | 30 | 1.750 | 0.500 | 1.6 | 6.0 | 1802.6 ± 160.3 |
|
|