Isolation, Purification, and Characterization of Xylanase Produced by a New Species of Bacillus in Solid State Fermentation

Table 1

Morphological, physiological, and biochemical characteristics of the isolate.

Tests	Results	Tests	Results
Colony morphology		Growth on NaCl (%)
Shape	Circular	2.0	−
Margin	Regular	4.0	−
Elevation	Raised	6.0	+
Consistency	Moist	8.0	+
Color	Pale yellow	10.0	−
Opacity	Opaque	Anaerobic Growth	Facultative
Gram nature	Gram Positive	Utilization of carbohydrates
Shape of the cell	Long rods	Xylose	+
Motility	Sluggish Motile	Lactose	+
Endospore position	Central	Mannitol	−
Growth at temperatures		Arabinose	−
10°C	−	Sucrose	−
25°C	−	Glucose	−
30°C	+	Fructose	+
37°C	+	Melibiose	−
45°C	+++	Starch hydrolysis	+
60°C	+	Gelatin hydrolysis	+
70°C	–	Urea hydrolysis	−
Growth at pH		Esculin hydrolysis	−
5.0	−	Casein hydrolysis	−
6.0	−	Tween 20 hydrolysis	+
7.0	+	Catalase test	+
8.0	++	Oxidase test	+
10.0	−	Nitrate reduction	+
		H2S production	−

+: Positive; −: Negative.