Expression of Four Methionine Sulfoxide Reductases in Staphylococcus aureus
Figure 3
Expression of the msr(A1/B), msrA2, and msrA3 loci in S. aureus SH1000 under different environmental stress conditions. Cultures of S. aureus SH1000 msr(A1/B)P-lacZ, msrA2P-lacZ, and msrA3P-lacZ reporter strains were grown to OD600 of 0.3 at 37°C and treated separately with H2O2 (15 mM) (2), oxacillin (1.2 μg/mL) (3), pH 5.0 (4), pH 9.0 (5), and TSB with 1.5 M added NaCl (6) for 1 h. β-galactosidase activity (lighter bar) and growth (OD600) (darker bar) were subsequently determined. β-galactosidase activity and growth of cells in TSB control are represented in bars 1. Values indicate averages of data from three independent experiments ± standard deviation (SD).
