Salivaricin G32, a Homolog of the Prototype Streptococcus pyogenes Nisin-Like Lantibiotic SA-FF22, Produced by the Commensal Species Streptococcus salivarius
Figure 1
Reversed phase fractionation of salivaricin G32 preparations. (a) Representative C8 reversed phase fractionation of concentrated XAD-2 fractions containing inhibitory activity against indicator organism Micrococcus luteus. Elution of the column was with a gradient of 18–30% acetonitrile at a flow rate of 1 mL·min−1 with detection of absorbance at 214 nm. (b) C18 reversed phase fractionation of pooled C8 reversed phase fractionated samples having inhibitory activity as indicated in panel A by the solid bar labelled 1. Elution was with isocratic 45% acetonitrile (containing 0.1% TFA) over 60 minutes at a flow rate of 0.7 mL·min−1. Active fractions (solid bar 2) from multiple runs were pooled then analysed by MALDI-TOF MS as indicated by the grey insert.
