Formulation and Statistical Optimization of Culture Medium for Improved Production of Antimicrobial Compound by Streptomyces sp. JAJ06

Table 4

Box-Behnken design matrix along with the experimental and predicted responses (antibiotic activity) of Streptomyces sp. JAJ06.

Runs	Variables/coded values			Antibiotic activity (U/mL) ± SEM
	Starch	KBr	CaCO3	Experimental	Predicted
1	−	−	0	110.00±10	108.32
2	+	−	0	140.00±5	136.25
3	−	+	0	120.00±10	123.75
4	+	+	0	148.30±2.9	149.97
5	−	0	−	130.00±10	127.29
6	+	0	−	150.00±10	149.36
7	−	0	+	110.00±0	110.64
8	+	0	+	140.00±10	142.71
9	0	−	−	120.00±0	124.39
10	0	+	−	138.30±7.6	137.26
11	0	−	+	110.00±10	111.04
12	0	+	+	131.70±2.9	127.31
13	0	0	0	163.30±5.8	165.34
14	0	0	0	168.30±2.9	165.34
15	0	0	0	166.70±5.8	165.34
16	0	0	0	166.70±5.8	165.34
17	0	0	0	161.70±7.6	165.34