Research Article
Efficient Degradation of Feather by Keratinase Producing Bacillus sp.
Table 1
Morphological and biochemical characterization of BF isolates.
| | Character | Test | Isolates | | BF11 | BF21 |
| | Growth at (°C) | 4 | − | − | | 10 | − | − | | 27 | + | + | | 37 | + | + | | 45 | + | + | | 55 | + | + | | 65 | − | − | | 4 | − | − |
| | Growth at different pH | 4.5 | − | − | | 5.5 | + | + | | 6.5 | + | + | | 7.5 | + | + | | 8.5 | + | + | | 9.5 | + | + | | 10.5 | − | − |
| | Biochemical tests | Indole | − | − | | MR | − | − | | VP | + | + | | Citrate | + | + |
| | Oxygen requirement | A | A |
| | Spore staining | + | + |
| | Motility | + | + |
| | Sugar utilization | Arabinose | − | − | | Glucose | + | + | | Sucrose | + | + | | Lactose | − | − | | Fructose | + | + | | Maltose | + | + | | Mannitol | + | + | | Xylose | − | − | | Glycerol | + | + | | Galactose | − | − | | Starch | + | + |
| | Enzymatic activity | Oxidase | − | − | | Catalase | + | + | | Urease | − | − | | Nitrate reduction | + | + | | Gelatinase | + | + | | Caseinase | + | + | | Amylase | + | + | | -Galactosidase | − | − |
| | Salt Tolerance (%) | 2 | + | + | | 5 | + | + | | 7 | + | + | | 10 | − | − |
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