Transcriptional Response of Selenopolypeptide Genes and Selenocysteine Biosynthesis Machinery Genes in Escherichia coli during Selenite Reduction

Table 1

Primers used for qRT-PCR.

Gene name	Forward primer	Reverse primer
fdnG	5^′-CCGAAGTGGGACCAGACCTA-3^′	5^′-TGACTTTGCCTTCATCCATCAT-3^′
fdoG	5^′-GCAGATCCGCAGGGTAACC-3^′	5^′-CTTAGTGCCGTCCCATTTCAG-3^′
fdhF	5^′-TTCTGTACGTGAAGCGACGAA-3^′	5^′-GCGATGGGCCATGTCATTAT-3^′
selA	5^′-CCGAAACGCGTTCCCTCTA-3^′	5^′-GGAGCTATCGCGCAATAAGC-3^′
selB	5^′-TCGCGTGCCTGGTTTTATC-3^′	5^′-GCCAGCATGTTGGAAAGAAACT-3^′
selD	5^′-ATAACGCTGGTGCCATTGC-3^′	5^′-GTAATGAAACCCGCGACGAT-3^′
sufS	5^′-CCGCGTAGCCATGACGATA-3^′	5^′-CAGAAACCGAGCCAGGTGAT-3^′
ybbB	5^′-CGTACGCGTGGGTAAAATCA-3^′	5^′-TGAGCGCCTGAACGAAGAGT-3^′
sbp	5^′-CCACCGTGTGACTGACGAAT-3^′	5^′-AGCGCCCACTGGAAACAG-3^′
thiP	5^′-AGGGTGCGGAAATCATCGT-3^′	5^′-GCTGTCGATTGGTGATTTTGG-3^′
iscsS	5^′-GGCCGGGTTACCAAAGGTT-3^′	5^′-GCGTGTTGCCGAGAAAATG-3^′
gor	5^′-ATCGGAAGAGAAGATTGTCGGTAT-3^′	5^′-CCCTGCAACATTTCGTCCAT-3^′
sodA	5^′-TCTCCGCTGATGGGTGAAG-3^′	5^′-CACATCCAGGCCCATAATCG-3^′
trxB	5^′-TGCCGGTCTGTTTGTTGCT-3^′	5^′-GCCCTTCGAAAATCGCAGTA-3^′
gutS	5^′-TGGAAATCGTCCCGTTGAA-3^′	5^′-GCCAGCACCATCAGGAGAAA-3^′
16S	5^′-TTTACGCCCAGTAATTCCGATT-3^′	5^′-CCAGCAGCCGCGGTAATA-3^′
glmM	5^′-AGCGGCGATCCACTTGAG-3^′	5^′-CCAGCGCAGCTTCAACCT-3^′
rfaP	5^′-GCGGATGCCCGTTTTG-3^′	5^′-CATCACTCAGGCGATGAATAGC-3^′
yegB	5^′-GGTTGGCATGGCGGTATTAA-3^′	5^′-TCAGCGGCGATAAACCTGTA-3^′
ydbA	5^′-CGCCATATGCGGGTGTAAA-3^′	5^′-GCATTGCGCTCCTGATAGC-3^′
ynbC	5^′-CGTCTGCGTGGTCTGTTTTTT-3^′	5^′-GCCGATCGTGGGTCAAATAG-3^′
phnI	5^′-TTTTGGCTTATTGGTGGATGTG-3′	5^′-TTACCGCAGAGCCGTTTTTT-3^′
nfi	5^′-CGTCGGCGAACTGCTGAT-3′	5^′-CGGCGGGTCGATAAAACC-3^′