Isolation of Bacillus sphaericus from Lombok Island, Indonesia, and Their Toxicity against Anopheles aconitus

Table 1

Characteristics of B. sphaericus isolated from Lombok Island.

Characteristics	Results/isolated	Standard
Cell
Form	Rod	Rod
Gram reaction	Positive	Positive
Size ()	3.0–5.0 × 0.5–0.75 m	1.5–5.0 × 0.6–1.0 m
Endospore	Positive	Positive
Endospore position	Terminal	Terminal/subterminal
Bulging endosporangium	Positive	Positive
Morphology
Form	Round	Round
Margin	Entire	Entire
Surface	Flat and smooth	Flat and smooth
Color	White-cream	Opaque (grown on nutrient agar)
Biochemical and physiological
Catalase	Positive	Positive
Starch hydrolysis	Negative	Negative
Acid production from sugar	Negative	Negative
Nitrate reduction	Negative	Negative
Urease	Positive	Positive
Oxidase	Positive	Positive
Aerobicity	Aerobe	Aerobe
Sensitivity to streptomycin	Resistant	Resistant
Sensitivity to chloramphenicol	Sensitive	Sensitive
Sensitivity to penicillin	Sensitive	Sensitive
Sensitivity to tetracycline	Sensitive	Sensitive
Sensitivity to amoxicillin	Sensitive	Sensitive
Sensitivity to vancomycin	Sensitive	Sensitive
Sensitivity to erythromycin	Sensitive	Sensitive
Sensitivity to gentamicin	Sensitive	Sensitive
Sensitivity to ciprofloxacin	Sensitive	Sensitive